Download PDF by Douglas E. Caldwell, Darren R. Korber, John R. Lawrence: Advances in Microbial Ecology

By Douglas E. Caldwell, Darren R. Korber, John R. Lawrence (auth.), K. C. Marshall (eds.)

ISBN-10: 1468476092

ISBN-13: 9781468476095

ISBN-10: 1468476114

ISBN-13: 9781468476118

Advances in Microbial Ecology was once demonstrated through the foreign Committee on Microbial Ecology (ICOME) to supply a car for in-depth, serious, or even provocative reports to stress contemporary tendencies within the vital box of microbial ecology. Advances in Microbial Ecology is now well-known as an enormous resource of infor­ mation and proposal either for practising and for potential microbial ecologists. such a lot studies showing in Advances were ready by way of leaders specifically components stick to­ ing invites issued through the Editorial Board. people are inspired, in spite of the fact that, to put up outlines of unsolicited contributions to any member of the Editorial Board for attention for book in Advances. With the e-book of quantity 12 of Advances in Microbial Ecology there'll be a metamorphosis of Editor and the whole Editorial Board. the present Editor needs to take this chance to thank the current Editorial Board, Ron Atlas, Bo Barker J~rgensen, and Gwyn Jones, in addition to previous contributors of the Board, for his or her tips and inspire­ ment through the years. the recent Editor of Advances in Microbial Ecology might be Gwyn Jones, with Bernhard Schink, Warwick F. Vincent, and David M. Ward as individuals of the Editorial Board. The outgoing Board want the hot Board each luck in continu­ ing the traditions verified by means of Martin Alexander, the founding Editor of Advances in Microbial Ecology. the themes featured in quantity 12 of Advances comprise a few relating to the meta­ bolic actions of micro organism; specifically, bioremediation of oil spills, via R. M. Atlas and R.

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16). P. jluorescens, V. parahaemolyticus, and C. crescentus attached to the upper and lower surfaces of flow cells at equivalent rates. Nonmotile mutants attached much less rapidly to the upper surface; however, cell settling and gravitational force facilitated cell attachment to lower surfaces (Fig. 16). 0 z..... 0 w ..... U) A- a: w u: w ..... 8 ~ Mot· bottom ~ o. 0 8T---------------------------, B Figure 16. Attachment of mot - and mot + Pseudomonas fluorescens to the walls of a glass flow cell at a laminar flow velocity of 40 ILm/sec (A).

30 Douglas E. Caldwell et al. flow velocities than substrate concentration (Caldwell and Lawrence, 1986). At concentrations exceeding 100 ppm glucose, P. fluorescens populations grew at their maximum rate independent of flow rate. However, at concentrations below 100 ppm, growth gradually stopped when flow stopped, and resumed again when flow resumed (Fig. 11). This suggested that cells growing within bioaggregates and biofilms easily become diffusion limited. Other studies have shown that a brief pulse of substrate resulted in the resumption of growth by starving cells after a I-hr lag period (Caldwell, 1987).

Both we and Harris (1991) have found that FITC is difficult to separate from RITC in dual-labeling experiments. Harris (1991) suggests the use of Texas red as an alternative to rhodamine in dual-labeling experiments. 6. Objective Lenses CLM places new demands on objective lens systems. The 3-D images which are fonned require that the axial (vertical) resolution of these lenses be considered as well as their traditional lateral resolution. In theory, the axial resolution of an objective lens is approximately one-half of the lateral resolution for the same lens, the 3-D image of a Confocal Laser Microscopy and Computer Image Analysis 21 diffraction-limited point light source being football-shaped (Linfoot and Wolfe, 1953; Inoue, 1990).

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Advances in Microbial Ecology by Douglas E. Caldwell, Darren R. Korber, John R. Lawrence (auth.), K. C. Marshall (eds.)

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