By A.H. Rose, J. Gareth Morris and D.W. Tempest (Eds.)
This quantity in a research-level sequence covers diversified facets of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial cellphone partitions. it really is meant to be of use to microbiologists, biochemists and biotechnologists. different comparable works during this sequence are volumes 29, 30 and 31.
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Additional info for Advances in Microbial Physiology, Vol. 24
VAN DIJKEN AND W. HARDER TABLE 6. 088 h-l) with methylamine as the nitrogen source and after transfer to ammonium sulphate as the nitrogen source. 6 Oxidase activities are expressed as pnol 0 2 min-' (mg protein)-', catalase activity as AA2a min-' (mg protein)-'. The number of peroxisomes is given as the average number per section; volume fractions are expressed as percentage of the cytoplasmic volume. sporulation experiments with cells of H. , 1980b). Unexpectedly, as was shown in methanol-limited chemostat cultures, growth of H .
Ethanol exerts an even stronger repression and is also able to repress alcohol oxidase synthesis under carbon-limitingconditions. The intracellular concentration of the repressor of alcohol oxidase synthesis may additionally be determined by the availability of intracellular energy. Thus addition of methanol to a glucose- or glycerollimited culture of these yeasts may enhance the rate of synthesis of alcohol oxidase through a decrease in concentration of the intracellular repressor. This may be attributable to the fact that extra energy is generated from the methanol which could enhance the flow of intermediates into the direction of biosynthesis, thereby lowering the concentration of the intracellular pools.
The method is based on trapping of hydrogen peroxide by Ce3+ ions resulting in the formation of an electron-dense complex, probably cerium perhydroxide (Ce(0H)ZOOH). Aerobic incubations of glutaraldehyde-fixed sphaeroplasts of H. polymorpha with this reagent confirmed the results obtained after incubations of cells with diaminobenzidine and methanol in that substrate-dependent reaction products were exclusively present in the microbodies (Fig. 6a). Therefore, according to the definition of de Duve (1973) these organelles may be considered as peroxisomes.
Advances in Microbial Physiology, Vol. 24 by A.H. Rose, J. Gareth Morris and D.W. Tempest (Eds.)